Mohamed A. Rashed, Mahmoud H. Abou-Deif, Ayman H. Atta, Fawzia I. Mohamed and Fatma E. Mahmoud
Abstract
Eighteen Egyptian inbred lines of maize (Zea maize L.) were used in this study to fingerprinting and identify the thermotolerance inbreds. The protein fingerprints for 14 inbred lines were performed by grain water-soluble protein electeophoretic analysis. The protein electrophoretic patterns showed 16 bands. There were six common bands in all inbreds, while 10 bands showed differences between inbred lines. The bands identified 11 inbreds in distinct patterns indicating different
genotypes, while the three inbred lines K10, G342 and K62 gave the same pattern. The high temperature effect on 17 maize inbred lines exposed to 35°C and 45°C for 4 hours at 14-days old besides control (25°C) was studied. Several protein bands varied between low and intermediate molecular weights were induced after exposing to heat stress 35°C and 45°C. Seven bands of heat shock proteins with molecular weights of 54.6, 28.7, 24.3, 18.2, 18, 14.2 and 12.8 kDa appeared in ten inbred lines after exposing to 45°C which may be indication of thermo-tolerance. Meanwhile, four of the seven bands with low molecular weights (28.7, 24.3, 18.2 and 18 kDa) were also expressed in four inbred lines in response to thermal stress at 35°C. The results indicated that the ten maize inbred lines K1, K5, K7, K8, K10, K11, K51, K61, G307A and Rg18 have thermo-tolerance until 45°C due to the appearance of heat shock proteins to overcome the adverse effect of high temperature.
Keywords : Maize, heat shock, proteins, electrophoresis.
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