Francesca Raimondo, Davide Ticozzi Valerio, Fulvio Magni, Roberto Perego, Cristina Bianchi, Cecilia Sarto, Stefano Casellato, Ester Fasoli, Stefano Ferrero, Ingrid Cifola, Francesco Rocco, Marzia Galli Kienle, Paolo Mocarelli, Paolo Brambilla and Marina Pitto
Abstract:
Caveolin-1 and flotillin-1 belong to plasma membrane microdomains. They are characterized by peculiar lipid and protein composition and are involved in fundamental cellular events such as: signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addressed caveolin-1 and flotillin-1 expression in 30 human renal cell carcinoma (RCC) and adjacent normal kidney (ANK) samples by SDS-PAGE and immunoblotting with specific antibodies. Significant caveolin-1 and flotillin-1 over-expression was found in RCC tissues compared to ANK, and was confirmed by immunohistochemistry. Caveolin-1 and flotillin-1 protein levels were found by 1-D, 2-DE, and MS to be increased also in RCC microdomain-enriched subcellular fractions purified from paired RCC and ANK samples.
Key Words: Caveolin, Flotillin, mass spectrometry, renal cell carcinoma, subcellular proteomics, two-dimensional electrophoresis.
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